A sensitive and reproducible 96-well assay of uronic acid permitting a rapid processing of a number of samples with a very low consumption of reagents is described-for the determination of complex uronic acid-bearing polyanions such as hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparin. The sensitivity of the reaction was approx. 1 mug for glucuronic acid and 2 mug for complex polysaccharides, with a linear function of glucuronic acid concentration between 1 and 100 mug. The relative coefficient of variations ranged from 1.5 to 8.7% for the assay performed in the 96-well plate. These values were found to be lower than those obtained by the conventional procedure.

A 96-well assay for uronic acid carbazole reaction / M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 54:1(2003), pp. 59-61. [10.1016/S0144-8617(03)00144-9]

A 96-well assay for uronic acid carbazole reaction

MACCARI, Francesca;VOLPI, Nicola
2003

Abstract

A sensitive and reproducible 96-well assay of uronic acid permitting a rapid processing of a number of samples with a very low consumption of reagents is described-for the determination of complex uronic acid-bearing polyanions such as hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparin. The sensitivity of the reaction was approx. 1 mug for glucuronic acid and 2 mug for complex polysaccharides, with a linear function of glucuronic acid concentration between 1 and 100 mug. The relative coefficient of variations ranged from 1.5 to 8.7% for the assay performed in the 96-well plate. These values were found to be lower than those obtained by the conventional procedure.
2003
54
1
59
61
A 96-well assay for uronic acid carbazole reaction / M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 54:1(2003), pp. 59-61. [10.1016/S0144-8617(03)00144-9]
M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/310491
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