Two cell populations with different phenol-phenotypes were selected from leaf explants of the ozone-sensitive Vitis vinifera L. cv. Lambrusco salamino. Experimental exposure of these cell lines to a 2 h fumigation with 300 ppb ozone caused a differential response. Peroxidative injuries, evaluated on the basis of malondialdehyde production and at the ultrastructural level, were induced in phenol-rich (Ph+) but not in phenol-poorer (Ph-) cells. Apoplastic ascorbate-peroxidase (EC 1.11.1.11), to which a prevailing role in apoplastic H2O2 scavenging has been attributed, was substantially stimulated in Ph- cells during ozone exposure; guaiacol-peroxidase (EC 1.11.1.7) reached maximum values 12 h later. Only guaiacol-peroxidase was rapidly induced in Ph+ cells, whereas ascorbate-peroxidase was inhibited to some extent. Phenol-poor cells thus proved able to promptly activate the biphasic extracellular peroxidase(s) defence system against ozone induced oxidative stress. On the contrary, the phenol-rich cell type appeared to be a very sensitive target of ozone toxicity; this suggests that the sensitivity displayed by the whole leaf of the grape cultivar used as explant source might be conditioned by the constitutive presence of a great number of such cells.

Differential sensitivity to ozone in two selected cell lines from grape leaf / Sgarbi, Elisabetta; Bonatti, Piera; Baroni, Roberta; A., Lins. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - STAMPA. - 154:(1999), pp. 119-126.

Differential sensitivity to ozone in two selected cell lines from grape leaf

SGARBI, Elisabetta;BONATTI, Piera;BARONI, Roberta;
1999

Abstract

Two cell populations with different phenol-phenotypes were selected from leaf explants of the ozone-sensitive Vitis vinifera L. cv. Lambrusco salamino. Experimental exposure of these cell lines to a 2 h fumigation with 300 ppb ozone caused a differential response. Peroxidative injuries, evaluated on the basis of malondialdehyde production and at the ultrastructural level, were induced in phenol-rich (Ph+) but not in phenol-poorer (Ph-) cells. Apoplastic ascorbate-peroxidase (EC 1.11.1.11), to which a prevailing role in apoplastic H2O2 scavenging has been attributed, was substantially stimulated in Ph- cells during ozone exposure; guaiacol-peroxidase (EC 1.11.1.7) reached maximum values 12 h later. Only guaiacol-peroxidase was rapidly induced in Ph+ cells, whereas ascorbate-peroxidase was inhibited to some extent. Phenol-poor cells thus proved able to promptly activate the biphasic extracellular peroxidase(s) defence system against ozone induced oxidative stress. On the contrary, the phenol-rich cell type appeared to be a very sensitive target of ozone toxicity; this suggests that the sensitivity displayed by the whole leaf of the grape cultivar used as explant source might be conditioned by the constitutive presence of a great number of such cells.
1999
154
119
126
Differential sensitivity to ozone in two selected cell lines from grape leaf / Sgarbi, Elisabetta; Bonatti, Piera; Baroni, Roberta; A., Lins. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - STAMPA. - 154:(1999), pp. 119-126.
Sgarbi, Elisabetta; Bonatti, Piera; Baroni, Roberta; A., Lins
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/309733
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