HR-MAS NMR spectroscopy in the characterization of human tissues: Application to healthy gastric mucosa

The HR-MAS is an ideal technique for the investigation of intact tissue specimens (10-50 mg) and permits the obtainment of spectra with a resolution comparable to that observed in solution in a time that does not exceed a half of an hour for a routine analysis. The potentialities of HR-MAS NMR spectroscopy in the identification of the metabolites characterizing the healthy gastric mucosa are here presented. The direct 1D H-1 NMR spectra enables only few metabolites to be confidently assigned, and the use of selected 2D experiments strongly amplify the analytical effectiveness of the technique.