Aim of this report was to investigate the adenylate pool and the energy charge in human white blood cells exposed to increasing time (15, 30 and 60 mm) of HeNe laser treatment. EDTA treated human blood diluted 1:1 whith 0.88% KCI was added (1:5) with NaCI-dextran solution to allow sedimentation of red blood cells. 6 ml of white cells floating in the supernatant were layered on 3 ml of Lymphoprep in plastic tubes and each tube was centrifuged (from 50 to 5000 x g for 5 mm). Granulocytes were concentrated in the lower phase, whilst lymphocytes were in the intermediated phase. After further purification cytological homogeneity was tested by a cell counter. Granulocytes and lymphocytes were irradiated at +22°C with HeNe (Space, Valfivre equipment). On these population ATP was tested by luminometric procedure, the adenylate pool was separated by HPLC (Jasco) on neutralyzed perchloric extracts. ATP concentration increases in lymphocytes (+63.9%, p < 0.01) and in granulocytes (+25.0%, p < 0.05) after 60 mm irradiation. The adenylate pool (tested by HPLC) does not change significatively in lymphocytes or granulocytes after 30 mm irradiation, whilst in 60 mm irradiated lymphocytes and granulocytes a significative increment was observed in nucleotide concentration. No changes were observed in energy charge according to Atkinson.

Adenylate pool and energy charge in human lymphocytes and granulocytes irradiated at 632 nm (HeNe Laser) / Bolognani, L.; Venturelli, T.; Volpi, N.; Zirilli, O.. - 2391:(1995), pp. 587-593. (Intervento presentato al convegno Laser-Tissue Interaction VI 1995 tenutosi a usa nel 1995) [10.1117/12.209928].

Adenylate pool and energy charge in human lymphocytes and granulocytes irradiated at 632 nm (HeNe Laser)

Volpi N.;
1995

Abstract

Aim of this report was to investigate the adenylate pool and the energy charge in human white blood cells exposed to increasing time (15, 30 and 60 mm) of HeNe laser treatment. EDTA treated human blood diluted 1:1 whith 0.88% KCI was added (1:5) with NaCI-dextran solution to allow sedimentation of red blood cells. 6 ml of white cells floating in the supernatant were layered on 3 ml of Lymphoprep in plastic tubes and each tube was centrifuged (from 50 to 5000 x g for 5 mm). Granulocytes were concentrated in the lower phase, whilst lymphocytes were in the intermediated phase. After further purification cytological homogeneity was tested by a cell counter. Granulocytes and lymphocytes were irradiated at +22°C with HeNe (Space, Valfivre equipment). On these population ATP was tested by luminometric procedure, the adenylate pool was separated by HPLC (Jasco) on neutralyzed perchloric extracts. ATP concentration increases in lymphocytes (+63.9%, p < 0.01) and in granulocytes (+25.0%, p < 0.05) after 60 mm irradiation. The adenylate pool (tested by HPLC) does not change significatively in lymphocytes or granulocytes after 30 mm irradiation, whilst in 60 mm irradiated lymphocytes and granulocytes a significative increment was observed in nucleotide concentration. No changes were observed in energy charge according to Atkinson.
1995
Laser-Tissue Interaction VI 1995
usa
1995
2391
587
593
Bolognani, L.; Venturelli, T.; Volpi, N.; Zirilli, O.
Adenylate pool and energy charge in human lymphocytes and granulocytes irradiated at 632 nm (HeNe Laser) / Bolognani, L.; Venturelli, T.; Volpi, N.; Zirilli, O.. - 2391:(1995), pp. 587-593. (Intervento presentato al convegno Laser-Tissue Interaction VI 1995 tenutosi a usa nel 1995) [10.1117/12.209928].
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1248440
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact