Peritoneal mouse macrophages elicited by proteose-peptone (pMΦ) were treated in vitro with IFN-α, IFN-β, or IFN-γ in the presence or absence of cycloheximide (Cy), a reversible inhibitor of protein synthesis, and were assayed for cytolytic activity against tumor cells. Inhibition of protein synthesis during treatment of pMΦ with IFN-α or IFN-β prevented the development of cytotoxic activity. In contrast, IFN-γ was fully capable of inducing cytotoxic pMΦ in the presence of Cy. Moreover, pMΦ treated with mixtures of IFN in the presence of Cy were activated for cytotoxicity only by IFN-γ together with IFN-α or IFN-β, but not by IFN-α plus IFN-β. These results indicate that the activation of pMΦ by IFN-γ is independent of new protein synthesis, whereas the activation of pMΦ by IFN-α and/or IFN-β requires active protein synthesis, suggesting that the mechanism of induction of cytotoxic pMΦ by IFN-γ differs from that by the other types of IFN.

Requirement for protein synthesis for induction of macrophage tumoricidal activity by IFN-α and IFN-β but not by IFN-γ / Blasi, E.; Herberman, R. B.; Varesio, L.. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 132:6(1984), pp. 3226-3228.

Requirement for protein synthesis for induction of macrophage tumoricidal activity by IFN-α and IFN-β but not by IFN-γ

Blasi E.;
1984

Abstract

Peritoneal mouse macrophages elicited by proteose-peptone (pMΦ) were treated in vitro with IFN-α, IFN-β, or IFN-γ in the presence or absence of cycloheximide (Cy), a reversible inhibitor of protein synthesis, and were assayed for cytolytic activity against tumor cells. Inhibition of protein synthesis during treatment of pMΦ with IFN-α or IFN-β prevented the development of cytotoxic activity. In contrast, IFN-γ was fully capable of inducing cytotoxic pMΦ in the presence of Cy. Moreover, pMΦ treated with mixtures of IFN in the presence of Cy were activated for cytotoxicity only by IFN-γ together with IFN-α or IFN-β, but not by IFN-α plus IFN-β. These results indicate that the activation of pMΦ by IFN-γ is independent of new protein synthesis, whereas the activation of pMΦ by IFN-α and/or IFN-β requires active protein synthesis, suggesting that the mechanism of induction of cytotoxic pMΦ by IFN-γ differs from that by the other types of IFN.
1984
132
6
3226
3228
Requirement for protein synthesis for induction of macrophage tumoricidal activity by IFN-α and IFN-β but not by IFN-γ / Blasi, E.; Herberman, R. B.; Varesio, L.. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 132:6(1984), pp. 3226-3228.
Blasi, E.; Herberman, R. B.; Varesio, L.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1247235
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 42
social impact