In Drosophila melanogaster the single male chromosome X undergoes an average twofold transcriptional upregulation for balancing the transcriptional output between sexes. Previous literature hypothesised that a global change in chromosome structure may accompany this process. However, recent studies based on Hi-C failed to detect these differences. Here we show that global conformational differences are specifically present in the male chromosome X and detectable using Hi-C data on sex-sorted embryos, as well as male and female cell lines, by leveraging custom data analysis solutions. We find the male chromosome X has more mid-/long-range interactions. We also identify differences at structural domain boundaries containing BEAF-32 in conjunction with CP190 or Chromator. Weakening of these domain boundaries in male chromosome X co-localizes with the binding of the dosage compensation complex and its co-factor CLAMP, reported to enhance chromatin accessibility. Together, our data strongly indicate that chromosome X dosage compensation affects global chromosome structure.

Global chromatin conformation differences in the Drosophila dosage compensated chromosome X / Pal, K.; Forcato, M.; Jost, D.; Sexton, T.; Vaillant, C.; Salviato, E.; Mazza, E. M. C.; Lugli, E.; Cavalli, G.; Ferrari, F.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 10:1(2019), pp. 1-16. [10.1038/s41467-019-13350-8]

Global chromatin conformation differences in the Drosophila dosage compensated chromosome X

Forcato M.;
2019

Abstract

In Drosophila melanogaster the single male chromosome X undergoes an average twofold transcriptional upregulation for balancing the transcriptional output between sexes. Previous literature hypothesised that a global change in chromosome structure may accompany this process. However, recent studies based on Hi-C failed to detect these differences. Here we show that global conformational differences are specifically present in the male chromosome X and detectable using Hi-C data on sex-sorted embryos, as well as male and female cell lines, by leveraging custom data analysis solutions. We find the male chromosome X has more mid-/long-range interactions. We also identify differences at structural domain boundaries containing BEAF-32 in conjunction with CP190 or Chromator. Weakening of these domain boundaries in male chromosome X co-localizes with the binding of the dosage compensation complex and its co-factor CLAMP, reported to enhance chromatin accessibility. Together, our data strongly indicate that chromosome X dosage compensation affects global chromosome structure.
2019
10
1
1
16
Global chromatin conformation differences in the Drosophila dosage compensated chromosome X / Pal, K.; Forcato, M.; Jost, D.; Sexton, T.; Vaillant, C.; Salviato, E.; Mazza, E. M. C.; Lugli, E.; Cavalli, G.; Ferrari, F.. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 10:1(2019), pp. 1-16. [10.1038/s41467-019-13350-8]
Pal, K.; Forcato, M.; Jost, D.; Sexton, T.; Vaillant, C.; Salviato, E.; Mazza, E. M. C.; Lugli, E.; Cavalli, G.; Ferrari, F.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1188769
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