Aphanizomenon flos-aquae (AFA) cyanobacteria from Klamath Lake (Oregon) are considered a “superfood”, due to their broad nutritional profile that has proved to have health-enhancing properties. AFA metabolome is quite complex. Here, we present a study that, combining multinuclear 1H, 31P and 13C Nuclear Magnetic Resonance (NMR) spectroscopy and high-resolution mass spectrometry, led to the detection of uncommon phosphorylated metabolites in AFA. We focused our attention on 31P NMR signals at 20 ppm, a chemical shift that usually points to the presence of phosphonates. The molecules contributing to 20 ppm 31P NMR signals revealed, instead, to be nucleoside 2’,3’-cyclic monophosphates. These metabolites were fully characterized by multinuclear 1H, 31P and 13C NMR spectroscopy and high-resolution mass spectrometry.

Nucleoside 2’,3’-cyclic monophosphates in Aphanizomenon flos-aquae detected through nuclear magnetic resonance and mass spectrometry / Zambon, A.; Righi, V.; Parenti, F.; Libertini, E.; Rossi, M. C.; Mucci, A.. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 1520-5118. - 67:(2019), pp. 12780-12785. [10.1021/acs.jafc.9b05991]

Nucleoside 2’,3’-cyclic monophosphates in Aphanizomenon flos-aquae detected through nuclear magnetic resonance and mass spectrometry

A. Zambon;F. Parenti;E. Libertini;M. C. Rossi;A. Mucci
2019

Abstract

Aphanizomenon flos-aquae (AFA) cyanobacteria from Klamath Lake (Oregon) are considered a “superfood”, due to their broad nutritional profile that has proved to have health-enhancing properties. AFA metabolome is quite complex. Here, we present a study that, combining multinuclear 1H, 31P and 13C Nuclear Magnetic Resonance (NMR) spectroscopy and high-resolution mass spectrometry, led to the detection of uncommon phosphorylated metabolites in AFA. We focused our attention on 31P NMR signals at 20 ppm, a chemical shift that usually points to the presence of phosphonates. The molecules contributing to 20 ppm 31P NMR signals revealed, instead, to be nucleoside 2’,3’-cyclic monophosphates. These metabolites were fully characterized by multinuclear 1H, 31P and 13C NMR spectroscopy and high-resolution mass spectrometry.
2019
8-nov-2019
67
12780
12785
Nucleoside 2’,3’-cyclic monophosphates in Aphanizomenon flos-aquae detected through nuclear magnetic resonance and mass spectrometry / Zambon, A.; Righi, V.; Parenti, F.; Libertini, E.; Rossi, M. C.; Mucci, A.. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 1520-5118. - 67:(2019), pp. 12780-12785. [10.1021/acs.jafc.9b05991]
Zambon, A.; Righi, V.; Parenti, F.; Libertini, E.; Rossi, M. C.; Mucci, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1185084
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