Milk and dairy products have long traditions in human nutrition since they are a rich source of nutrients such lipids, proteins, amino acids, vitamins and minerals. Nowadays, milk may also be considered as a source of health-promoting compounds. Bioactive peptides deriving from milk proteins may play an important role in the prevention and treatment of metabolic syndrome and its complications [1]. This study aimed to characterize the peptidomic profile of in vitro digested skimmed bovine, camel, goat and sheep milk. Milk samples were in vitro digested following the harmonized INFOGEST protocol [2]. The peptide fractions were extracted from fully digested milk by ultrafiltration (cut-off 3 kDa) and the permeate were characterized by nanoflow-LC-ESI-QTOF MS/MS analysis. Digestibility and biological activities of the peptide fractions were also determined at the end of the digestion. The results showed a faster and more efficient gastric and duodenal degradation of goat milk proteins than camel, bovine and sheep milk. More than 100 peptides were identified in the permeate of digested milk, most of them arising from beta-casein. Goat, sheep and bovine milk showed the highest similarity in peptide sequences than camel milk. Peptides with previously demonstrated biological activities were found in the permeate of all the milk samples after in vitro digestion. Biological activities analysis showed that bovine milk peptides were the most effective in scavenging hydroxyl radical and in the inhibition lipid peroxidation. Bovine milk was also the best source of DPPIV-inhibitory peptides (IC50=6.87 mg of peptides/mL) whereas sheep milk was the best source of ACE-inhibitory peptides (IC50=625.4 μg of peptides/mL). The quantitative diversity in the identified bioactive peptides explained the observed differences in the biological activities.

PEPTIDOMIC PROFILE OF IN VITRO DIGESTED BOVINE, CAMEL, GOAT AND SHEEP MILK / Tagliazucchi, Davide; Martini, Serena; Sherif, Shamsia; Ahmed, Helal; Conte, Angela. - (2017). (Intervento presentato al convegno 59th National Meeting of the Italian Society of Biochemistry and Molecular Biology tenutosi a Caserta nel 20-22 September 2017).

PEPTIDOMIC PROFILE OF IN VITRO DIGESTED BOVINE, CAMEL, GOAT AND SHEEP MILK

TAGLIAZUCCHI, Davide;MARTINI, SERENA;CONTE, Angela
2017

Abstract

Milk and dairy products have long traditions in human nutrition since they are a rich source of nutrients such lipids, proteins, amino acids, vitamins and minerals. Nowadays, milk may also be considered as a source of health-promoting compounds. Bioactive peptides deriving from milk proteins may play an important role in the prevention and treatment of metabolic syndrome and its complications [1]. This study aimed to characterize the peptidomic profile of in vitro digested skimmed bovine, camel, goat and sheep milk. Milk samples were in vitro digested following the harmonized INFOGEST protocol [2]. The peptide fractions were extracted from fully digested milk by ultrafiltration (cut-off 3 kDa) and the permeate were characterized by nanoflow-LC-ESI-QTOF MS/MS analysis. Digestibility and biological activities of the peptide fractions were also determined at the end of the digestion. The results showed a faster and more efficient gastric and duodenal degradation of goat milk proteins than camel, bovine and sheep milk. More than 100 peptides were identified in the permeate of digested milk, most of them arising from beta-casein. Goat, sheep and bovine milk showed the highest similarity in peptide sequences than camel milk. Peptides with previously demonstrated biological activities were found in the permeate of all the milk samples after in vitro digestion. Biological activities analysis showed that bovine milk peptides were the most effective in scavenging hydroxyl radical and in the inhibition lipid peroxidation. Bovine milk was also the best source of DPPIV-inhibitory peptides (IC50=6.87 mg of peptides/mL) whereas sheep milk was the best source of ACE-inhibitory peptides (IC50=625.4 μg of peptides/mL). The quantitative diversity in the identified bioactive peptides explained the observed differences in the biological activities.
2017
59th National Meeting of the Italian Society of Biochemistry and Molecular Biology
Caserta
20-22 September 2017
Tagliazucchi, Davide; Martini, Serena; Sherif, Shamsia; Ahmed, Helal; Conte, Angela
PEPTIDOMIC PROFILE OF IN VITRO DIGESTED BOVINE, CAMEL, GOAT AND SHEEP MILK / Tagliazucchi, Davide; Martini, Serena; Sherif, Shamsia; Ahmed, Helal; Conte, Angela. - (2017). (Intervento presentato al convegno 59th National Meeting of the Italian Society of Biochemistry and Molecular Biology tenutosi a Caserta nel 20-22 September 2017).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1146090
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