An analytical procedure has been developed for the assay of adenosine and some metabolites in brain tissue extracts of rats. This paper reports the extraction method, the technique adopted to avoid enzymatic transformations and the chromatographic conditions for the identification and quantitation of these nucleosides and bases. Peaks in the chromatograms of brain tissue extracts were identified by retention times, absorbance ratios of reference compounds and by enzymatic peak-shift.
A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF ADENOSINE AND SOME METABOLITES IN THE BRAIN-TISSUE OF RATS / Gamberini, G; Ferioli, Valeria; Zanoli, Paola; Zeneroli, Maria Luisa; Rustichelli, Cecilia; Baraldi, Mario. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 34:(1992), pp. 563-567.
A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF ADENOSINE AND SOME METABOLITES IN THE BRAIN-TISSUE OF RATS
FERIOLI, Valeria;ZANOLI, Paola;ZENEROLI, Maria Luisa;RUSTICHELLI, Cecilia;BARALDI, Mario
1992
Abstract
An analytical procedure has been developed for the assay of adenosine and some metabolites in brain tissue extracts of rats. This paper reports the extraction method, the technique adopted to avoid enzymatic transformations and the chromatographic conditions for the identification and quantitation of these nucleosides and bases. Peaks in the chromatograms of brain tissue extracts were identified by retention times, absorbance ratios of reference compounds and by enzymatic peak-shift.
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