The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AABrelated OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification.

Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria / Vacchini, V; Gonella, E; Crotti, E; Prosdocimi, Em; Mazzetto, F; Chouaia, B; Callegari, M; Mapelli, M; Mandrioli, Mauro; Alma, A; Daffonchio, D.. - In: ENVIRONMENTAL MICROBIOLOGY REPORTS. - ISSN 1758-2229. - STAMPA. - 9:(2017), pp. 91-103. [10.1111/1758-2229.12505]

Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria

MANDRIOLI, Mauro;
2017

Abstract

The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AABrelated OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification.
2017
13-mar-2017
9
91
103
Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria / Vacchini, V; Gonella, E; Crotti, E; Prosdocimi, Em; Mazzetto, F; Chouaia, B; Callegari, M; Mapelli, M; Mandrioli, Mauro; Alma, A; Daffonchio, D.. - In: ENVIRONMENTAL MICROBIOLOGY REPORTS. - ISSN 1758-2229. - STAMPA. - 9:(2017), pp. 91-103. [10.1111/1758-2229.12505]
Vacchini, V; Gonella, E; Crotti, E; Prosdocimi, Em; Mazzetto, F; Chouaia, B; Callegari, M; Mapelli, M; Mandrioli, Mauro; Alma, A; Daffonchio, D.
File in questo prodotto:
File Dimensione Formato  
reprint_Vacchini_et_al-2017-Environmental_Microbiology_Reports.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 532.79 kB
Formato Adobe PDF
532.79 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
POST_PRINT_1088-5918-1-PB.pdf

Open access

Tipologia: Versione dell'autore revisionata e accettata per la pubblicazione
Dimensione 332.73 kB
Formato Adobe PDF
332.73 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1129130
Citazioni
  • ???jsp.display-item.citation.pmc??? 23
  • Scopus 44
  • ???jsp.display-item.citation.isi??? 46
social impact