The aim of this study was to assess the production of extended spectrum beta-lactamases (ESBL) in 56 strains of Enterobacteriaceae, obtained from 100 rectal swabs of farm animals, and to evaluate the horizontal transfer capacity of the genetic determinants of resistance. The ESBL-positive strains were confirmed by phenotypic testing, confirmed by PCR and DNA sequence analysis. The localization of beta-lactamase genes was established by conjugation experiments. Of the 56 analyzed strains, 20 (36%) resulted positive for ESBL production by the double-disk synergy test, and belonged to Escherichia coli 15 (75%) and Klebsiella ozaenae 5 (25%) species. Molecular analysis showed that all ESBL-producing isolates possessed genes encoding for TEM-type enzymes and/or CTX-M. The conjugation assays yielded positive results, thus denoting a plasmidic localization of the genes. This study highlights the high percentage of ESBL-positive Enterobacteriaceae and the mobility of the responsible genes. Gene mobility implies highly negative consequences in terms of drug therapy because of the spread of antibiotic resistance.

Prevalence and characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae in food-producing animals in Northern Italy / Stefani, Sara; Giovanelli, I; Anacarso, Immacolata; Condo', Carla; Messi, Patrizia; De Niederhausern, Simona; Bondi, Moreno; Iseppi, Ramona; Sabia, Carla. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 37:4(2014), pp. 551-555.

Prevalence and characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae in food-producing animals in Northern Italy

STEFANI, SARA;ANACARSO, Immacolata;CONDO', CARLA;MESSI, Patrizia;DE NIEDERHAUSERN, Simona;BONDI, Moreno;ISEPPI, Ramona;SABIA, Carla
2014

Abstract

The aim of this study was to assess the production of extended spectrum beta-lactamases (ESBL) in 56 strains of Enterobacteriaceae, obtained from 100 rectal swabs of farm animals, and to evaluate the horizontal transfer capacity of the genetic determinants of resistance. The ESBL-positive strains were confirmed by phenotypic testing, confirmed by PCR and DNA sequence analysis. The localization of beta-lactamase genes was established by conjugation experiments. Of the 56 analyzed strains, 20 (36%) resulted positive for ESBL production by the double-disk synergy test, and belonged to Escherichia coli 15 (75%) and Klebsiella ozaenae 5 (25%) species. Molecular analysis showed that all ESBL-producing isolates possessed genes encoding for TEM-type enzymes and/or CTX-M. The conjugation assays yielded positive results, thus denoting a plasmidic localization of the genes. This study highlights the high percentage of ESBL-positive Enterobacteriaceae and the mobility of the responsible genes. Gene mobility implies highly negative consequences in terms of drug therapy because of the spread of antibiotic resistance.
2014
37
4
551
555
Prevalence and characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae in food-producing animals in Northern Italy / Stefani, Sara; Giovanelli, I; Anacarso, Immacolata; Condo', Carla; Messi, Patrizia; De Niederhausern, Simona; Bondi, Moreno; Iseppi, Ramona; Sabia, Carla. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 37:4(2014), pp. 551-555.
Stefani, Sara; Giovanelli, I; Anacarso, Immacolata; Condo', Carla; Messi, Patrizia; De Niederhausern, Simona; Bondi, Moreno; Iseppi, Ramona; Sabia, Carla
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1127773
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