BACKGROUND: Urine are easily accessible and relatively simple to process and uronic acid-bearing glycosaminoglycans (UA-GAGs) may serve as biomarkers for several diseases, like for mucopolysaccharidosis. METHODS: We report a study from a large cohort of healthy newborns of 2-3days to have a basic profile of total content of urinary UA-GAGs, their composition and structural signatures utilizing a rapid extractive method and sensitive separation of enzymatic released disaccharides by capillary electrophoresis-light induced fluorescence. Results were also compared with those obtained from normal adult subjects. RESULTS: A total of UA-GAGs content of ~35μg/mg creatinine was observed in 331 newborns versus 1.5μg/mg creatinine of adult urine composed of ~90% chondroitin sulfate (CS), ~7% heparan sulfate (HS) and ~3% hyaluronic acid (HA). No significant differences were observed with adults. Specific ratios between the main CS disaccharides were informative of a significant greater 4-sulfation and charge density for newborn compared to adults. The HS from newborn urine was mainly composed by the non-sulfated (~64%) and mono-sulfated (~28%) disaccharides. No significant differences were observed versus adult urine. CONCLUSIONS: The present method is able to measure changes in UA-GAG composition and their structure independently of the age of subjects and rapidly applicable to the newborn diagnosis without necessity to have creatinine levels. Moreover, modifications in charge density values as well as the presence of sulfate groups in specific positions may be indicative of altered conditions. Copyright © 2016 Elsevier B.V. All rights reserved.

Total and single species of uronic acid-bearing glycosaminoglycans in urine of newborns of 2-3 days of age for early diagnosis application / Maccari, Francesca; Galeotti, Fabio; Lucia, Zampini; Lucia, Padella; Rosella, Tomanin; Daniela, Concolino; Agata, Fiumara; Tiziana, Galeazzi; Orazio, Gabrielli; Giovanni, Coppa; Volpi, Nicola. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 463:(2016), pp. 67-72. [10.1016/j.cca.2016.10.012]

Total and single species of uronic acid-bearing glycosaminoglycans in urine of newborns of 2-3 days of age for early diagnosis application

MACCARI, Francesca;GALEOTTI, FABIO;VOLPI, Nicola
2016

Abstract

BACKGROUND: Urine are easily accessible and relatively simple to process and uronic acid-bearing glycosaminoglycans (UA-GAGs) may serve as biomarkers for several diseases, like for mucopolysaccharidosis. METHODS: We report a study from a large cohort of healthy newborns of 2-3days to have a basic profile of total content of urinary UA-GAGs, their composition and structural signatures utilizing a rapid extractive method and sensitive separation of enzymatic released disaccharides by capillary electrophoresis-light induced fluorescence. Results were also compared with those obtained from normal adult subjects. RESULTS: A total of UA-GAGs content of ~35μg/mg creatinine was observed in 331 newborns versus 1.5μg/mg creatinine of adult urine composed of ~90% chondroitin sulfate (CS), ~7% heparan sulfate (HS) and ~3% hyaluronic acid (HA). No significant differences were observed with adults. Specific ratios between the main CS disaccharides were informative of a significant greater 4-sulfation and charge density for newborn compared to adults. The HS from newborn urine was mainly composed by the non-sulfated (~64%) and mono-sulfated (~28%) disaccharides. No significant differences were observed versus adult urine. CONCLUSIONS: The present method is able to measure changes in UA-GAG composition and their structure independently of the age of subjects and rapidly applicable to the newborn diagnosis without necessity to have creatinine levels. Moreover, modifications in charge density values as well as the presence of sulfate groups in specific positions may be indicative of altered conditions. Copyright © 2016 Elsevier B.V. All rights reserved.
2016
11-ott-2016
463
67
72
Total and single species of uronic acid-bearing glycosaminoglycans in urine of newborns of 2-3 days of age for early diagnosis application / Maccari, Francesca; Galeotti, Fabio; Lucia, Zampini; Lucia, Padella; Rosella, Tomanin; Daniela, Concolino; Agata, Fiumara; Tiziana, Galeazzi; Orazio, Gabrielli; Giovanni, Coppa; Volpi, Nicola. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 463:(2016), pp. 67-72. [10.1016/j.cca.2016.10.012]
Maccari, Francesca; Galeotti, Fabio; Lucia, Zampini; Lucia, Padella; Rosella, Tomanin; Daniela, Concolino; Agata, Fiumara; Tiziana, Galeazzi; Orazio, Gabrielli; Giovanni, Coppa; Volpi, Nicola
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0009898116304090-main.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 844.46 kB
Formato Adobe PDF
844.46 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1118096
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
social impact