Biocompatibility in hemodialysis is now regarded as a multifactorial problem and dialysate represents a main risk. Pyrogenic fractions mostly coming from gram-negative bacteria easily pass through dialysis membrane, either by backdiffusion or by backfiltration, and induce blood cell activation. To demonstrate the long-term efficiency of a 2 M2 polyamide ultrafilter in producing a pyrogen free solution, we used an experimental circuit ultrafiltering for 240 hours (500 ml/min) a bicarbonate dialysate contaminated (5 to 48 EU/ml) by a Pseudomonas aeruginosa filtrate. The efficiency was monitored by LAL-test and IL-1 PBMC so to detect not only lipid A containing endotoxins but also other cytokines inducing bacterial fractions. At the post-ultrafilter sampling port the LAL-test was <0.005 to 0.034 EU/ml; IL-1 PBMC was below the detection limit (20 pg/ml) being 27 to 63 pg/ml at the pre-ultrafilter level. Polyamide ultrafiltration represents an efficient system to obtain an endotoxin-free dialysate and a single filter works up to 240 hours.

ULTRAFILTRATION AND ENDOTOXIN REMOVAL FROM DIALYSIS FLUIDS / Difelice, A; Cappelli, Gianni; Facchini, F; Tetta, C; Cornia, F; Aimo, G; Lusvarghi, E.. - In: KIDNEY INTERNATIONAL. - ISSN 0085-2538. - STAMPA. - 43:(1993), pp. S 201-S 204.

ULTRAFILTRATION AND ENDOTOXIN REMOVAL FROM DIALYSIS FLUIDS

CAPPELLI, Gianni;
1993

Abstract

Biocompatibility in hemodialysis is now regarded as a multifactorial problem and dialysate represents a main risk. Pyrogenic fractions mostly coming from gram-negative bacteria easily pass through dialysis membrane, either by backdiffusion or by backfiltration, and induce blood cell activation. To demonstrate the long-term efficiency of a 2 M2 polyamide ultrafilter in producing a pyrogen free solution, we used an experimental circuit ultrafiltering for 240 hours (500 ml/min) a bicarbonate dialysate contaminated (5 to 48 EU/ml) by a Pseudomonas aeruginosa filtrate. The efficiency was monitored by LAL-test and IL-1 PBMC so to detect not only lipid A containing endotoxins but also other cytokines inducing bacterial fractions. At the post-ultrafilter sampling port the LAL-test was <0.005 to 0.034 EU/ml; IL-1 PBMC was below the detection limit (20 pg/ml) being 27 to 63 pg/ml at the pre-ultrafilter level. Polyamide ultrafiltration represents an efficient system to obtain an endotoxin-free dialysate and a single filter works up to 240 hours.
1993
43
S 201
S 204
ULTRAFILTRATION AND ENDOTOXIN REMOVAL FROM DIALYSIS FLUIDS / Difelice, A; Cappelli, Gianni; Facchini, F; Tetta, C; Cornia, F; Aimo, G; Lusvarghi, E.. - In: KIDNEY INTERNATIONAL. - ISSN 0085-2538. - STAMPA. - 43:(1993), pp. S 201-S 204.
Difelice, A; Cappelli, Gianni; Facchini, F; Tetta, C; Cornia, F; Aimo, G; Lusvarghi, E.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/11151
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