Hematopoiesis entails a series of hierarchically organized events that proceed throughout cell specification and terminates with cell differentiation. Commitment needs the transcription factors effort that, in concert with microRNAs, drives cell fate and responds to promiscuous patterns of gene expression by turning-on lineage-specific genes and repressing alternate lineage transcripts. We obtained microRNAs profiles from human CD34+ hematopoietic progenitor cells and in-vitro differentiated erythroblasts, megakaryoblasts, monoblasts and myeloblasts precursors, that we analyzed together with their gene expression profiles. The integrated analysis of microRNA-mRNA expression levels highlighted an inverse correlation between microRNAs specifically up-regulated in one single cell progeny and their putative target genes, which resulted down-regulated. Among the up-regulated lineage-enriched microRNAs, hsa-miR-299-5p emerged as having a role in controlling CD34+ progenitors fate, grown in multilineage culture conditions. Gain- and loss-of-function experiments revealed that hsa-miR-299-5p participates the regulation of hematopoietic progenitors fate, modulating megakaryocytic-granulocytic versus erythroid-monocytic differentiation.

Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoieis: role of hsa-mir- 299-5p in CD34+ progenitor cells commitment / Tenedini, Elena. - In: THE HUGO JOURNAL. - ISSN 1877-6566. - 4 (supplement 1):(2010), pp. 116-116. (Intervento presentato al convegno 14th Human Genome Meeting 2010 tenutosi a Le Corum, Montepellier, France nel 18-21 May 2010).

Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoieis: role of hsa-mir- 299-5p in CD34+ progenitor cells commitment

TENEDINI, Elena
2010

Abstract

Hematopoiesis entails a series of hierarchically organized events that proceed throughout cell specification and terminates with cell differentiation. Commitment needs the transcription factors effort that, in concert with microRNAs, drives cell fate and responds to promiscuous patterns of gene expression by turning-on lineage-specific genes and repressing alternate lineage transcripts. We obtained microRNAs profiles from human CD34+ hematopoietic progenitor cells and in-vitro differentiated erythroblasts, megakaryoblasts, monoblasts and myeloblasts precursors, that we analyzed together with their gene expression profiles. The integrated analysis of microRNA-mRNA expression levels highlighted an inverse correlation between microRNAs specifically up-regulated in one single cell progeny and their putative target genes, which resulted down-regulated. Among the up-regulated lineage-enriched microRNAs, hsa-miR-299-5p emerged as having a role in controlling CD34+ progenitors fate, grown in multilineage culture conditions. Gain- and loss-of-function experiments revealed that hsa-miR-299-5p participates the regulation of hematopoietic progenitors fate, modulating megakaryocytic-granulocytic versus erythroid-monocytic differentiation.
2010
14th Human Genome Meeting 2010
Le Corum, Montepellier, France
18-21 May 2010
4 (supplement 1)
116
116
Tenedini, Elena
Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoieis: role of hsa-mir- 299-5p in CD34+ progenitor cells commitment / Tenedini, Elena. - In: THE HUGO JOURNAL. - ISSN 1877-6566. - 4 (supplement 1):(2010), pp. 116-116. (Intervento presentato al convegno 14th Human Genome Meeting 2010 tenutosi a Le Corum, Montepellier, France nel 18-21 May 2010).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1114285
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