Aptamers are synthetic nucleic acid molecules that can bind biological targets in virtue of both their sequence and three-dimensional structure. Aptamers are selected using SELEX, Systematic Evolution of Ligands by EXponential enrichment, a technique that exploits aptamer-target binding affinity. The SELEX procedure, coupled with high-throughput sequencing (HT-SELEX), creates billions of random sequences capable of binding different epitopes on specific targets. Since this technique produces enormous amounts of data, computational analysis represents a critical step to screen and select the most biologically relevant sequences.
APTANI: a computational tool to select aptamers through sequence-structure motif analysis of HT-SELEX data / Caroli, Jimmy; Taccioli, C; De La Fuente, A; Serafini, P; Bicciato, Silvio. - In: BIOINFORMATICS. - ISSN 1367-4803. - ELETTRONICO. - 32:(2016), pp. 161-164. [10.1093/bioinformatics/btv545]
APTANI: a computational tool to select aptamers through sequence-structure motif analysis of HT-SELEX data
CAROLI, JIMMY;BICCIATO, Silvio
2016
Abstract
Aptamers are synthetic nucleic acid molecules that can bind biological targets in virtue of both their sequence and three-dimensional structure. Aptamers are selected using SELEX, Systematic Evolution of Ligands by EXponential enrichment, a technique that exploits aptamer-target binding affinity. The SELEX procedure, coupled with high-throughput sequencing (HT-SELEX), creates billions of random sequences capable of binding different epitopes on specific targets. Since this technique produces enormous amounts of data, computational analysis represents a critical step to screen and select the most biologically relevant sequences.File | Dimensione | Formato | |
---|---|---|---|
btv545_online.pdf
Accesso riservato
Tipologia:
Versione pubblicata dall'editore
Dimensione
231.79 kB
Formato
Adobe PDF
|
231.79 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris