Superhighflux therapies for hemodialysis: ultrafiltrate proteomic profile and protein identification by on-chip elution.

between serum levels of symmetric dimethylarginine (SDMA) and N-terminal natriuretic propeptide B-type (NT-proBNP ) and the degree of estimated glomerular filtration rate (eGFR). Methods: This study involved 98 chronic kidney disease (CKD) patients and 44 renal transplant (RT) recipients. Measurement of SDMA was determined using ELISA (DLD Diagnostica GMBH) and NT-proBNP was determined with ELFA (bioMerieux). GFR was calculated according to the Modified Diet in Renal Disease (MDRD) equation. Results: To evaluate the changes of examined parameters according to renal function we stratified CKD and RT patients to GFR categories. All patients have moderate (GFR 30–59 ml/min/1.73 m2) to severe (GFR <30 mL/min/1.73 m2) impaired renal function. Both NT-proBNP and SDMA were significantly higher in CKD and RT patients with severe than moderate impaired renal function. In CKD group, NT-proBNP values rose from 88,09 ng/L in patients with moderate eGFR to 988,78 pg/L in patients with severe eGFR (P <0.001) and from 197,05 ng/L to 996,87 ng/L in RT group (P=0.003). Obtained values for SDMA between moderate and severe eGFR were 1,09 μmol/L vs 1,62 μmol/L in CKD group and 0,58 μmol/L vs 1,18 μmol/L in RT group (P <0.001). Conclusion: NT-proBNP and SDMA are usefull for detection of severity of renal disfunction in CKD patients and renal transplant recipients.