ŸDevelopment of a multi-analyte diagnostic tool (Automatic Laboratory Detector) for testing the presence of resistant pathogens to antibiotics through direct biomarker detection. Specifically OPTO-bacteria will be able to identify ESBL type enzymes and other known proteins produced by infectious microorganism resistant to currently prescribed beta-lactam antibiotic and others (drug resistance biomarkers). As a first application ESBLs will be identified in biofluid without prior bacterial growth. After appropriate sample treatment and bacteria breaking, the intracellular proteins will be optobact released into the biofluid and the optical-fiber device, functionalized with the appropriate ligand, will recognize/bind the specific enzyme. • Development of a multi-analyte diagnostic tool (Automatic Laboratory Detector) for testing the presence of resistant pathogens to antibiotic prescription through rapid bacterial growth detection. In this case the biological sample would be a pure culture of bacteria. The highly sensitive technology allows us to monitoring over a very short time one (6-8 hours) the specific parameter (parameter G) related to the number of bacteria that are growing into the biological sample in the presence of specific antibiotics. The variation of parameter G within a short time frame will indicate the sensitivity or not to the prescribed drug. •Preparation of a robotic system (ALD) to be used in hospital as diagnostic tool allowing a low invasive and low cost detection of resistant pathogenic infections overexpressing beta- lactamase and other biomarker of drug resistance. The instrument will be equipped with disposable detection tips, friendly handling, will provide high accuracy, high sensitivity and high resolution. •In vitro validation and in pilot clinical setting studies of the multianalyte device (ALD) using biofluid with different level of complexity. • Design and synthesis of novel specific ligands (probes) able to recognize clinically relevant BLs and other known fundamental biomarkers.
|Titolo:||Capacity Multianalyte Automatic System for the Detection of Drug Resistant Bacteria. FP7 Capacity .Grant agreement no: 286998|
|Autori:||Maria, Paola Costi; Venturelli Alberto; Donatella, Tondi; Matteo, Santucci; Francesca, Spyrakis; Tatiana Vignudelli; Stefania Ferrari; Davide, Farina|
|Data di pubblicazione:||2012|
|Appare nelle tipologie:||Partecipazione a progetti di ricerca|
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